Figure 3. Results of High-Performance Liquid Chromatography, Gas Chromatography, and Mass Spectrometry of PC-SPES and the Estrogens Estrone, Estradiol, and Diethylstilbestrol (DES).

The results of high-performance liquid chromatography, shown in Panels A and B, are representative of three experiments with two separate batches of PC-SPES. The results of gas chromatography and mass spectrometry show that peak X of PC-SPES in Panel B differs from the peak representing diethylstilbestrol in Panel A. Gas chromatograms of diethylstilbestrol (Panel C) and peak X of PC-SPES (Panel D) are shown along with in-line mass spectrometric analysis of diethylstilbestrol (inset in Panel C) and peak Y of peak X (inset in Panel D). The mass spectrometer was set to scan particles with a mass-to-charge ratio (m/z) ranging from 17 to 600 every 0.6 second.

Figure 3: from: Clinical and Biologic Activity of an Estrogenic Herbal Combination (PC-SPES) in Prostate Cancer

Robert S. DiPaola, M.D., Huayan Zhang, M.D., George H. Lambert, M.D., Robert Meeker, B.S., Edward Licitra, Ph.D., Mohamed M. Rafi, Ph.D., Bao Ting Zhu, Ph.D., Heidi Spaulding, R.N., Susan Goodin, Pharm.D., Michel B. Toledano, M.D., William N. Hait, M.D., Ph.D., and Michael A. Gallo, Ph.D.

New England Journal of Medicine, Volume 339:785-791 September 17, 1998 Number 12

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